■ 基本信息
別名: | pETBlue2 |
啟動子: | T7 |
復制子: | pBR322 |
終止子: | T7 terminator |
質粒分類: | 大腸桿菌載體；PET系列表達質粒 |
質粒大小: | 3653bp |
質粒標簽: | C-HSV，C-6×His |
原核抗性: | Amp |
真核抗性: | LacZ |
克隆菌株: | DH5a |
培養條件: | 37℃，有氧，LB |
表達宿主: | 大腸桿菌BL21(DE3)pLacI |
誘導方式: | IPTG或乳糖及其類似物 |
5'測序引物:
| T7:TAATACGACTCACTATAGGG
|
3'測序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標記: |
|
■ 質粒簡介
pETBlue-2是一個大腸桿菌pET系列表達質粒。The pETBlue?-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV?Tag? and His?Tag? sequences. The sequence is numbered from the first base of the T7 promoter sequence.
質粒只保證關鍵序列正確，不保證表達效果。
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK196pETBlue-2大腸表達質粒.txt
