■ 基本信息
別名: | pIRES-EGFP，pIRES2EGFP |
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 哺乳細胞，熒光蛋白報告載體 |
質粒大小: | 5308bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | pIRES2-EGFP.P5：GTAGGCGTGTACGGTGGGAG |
3'測序引物: | pIRES2-EGFP.P3：AACGCACACCGGCCTTATTC |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Kan |
真核抗性: | G418
|
熒光標記: | 綠色
|
■ 質粒簡介
pIRES2-EGFP contains the internal ribosome entry site (IRES; 1, 2) of the encephalomyocarditis virus (ECMV) between the MCS and the enhanced green fluorescent protein (EGFP) coding region.This permits both the gene of interest (cloned into the MCS) and the EGFP gene to be translated from a single bicistronic mRNA. pIRES2-EGFP is designed for the efficient selection (by flow cytometry or other methods) of transiently transfected mammalian cells expressing EGFP and the protein of interest. This vector can also be used to express EGFP alone or to obtain stably transfected cell lines without time-consuming drug and clonal selection.
質粒只保證關鍵序列正確，不保證表達效果。
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK427pIRES2-EGFP哺乳熒光質粒.txt
