■ 基本信息
別名: | 金黃葡萄球染體異位互補 |
原核抗性: | Amp |
| 克隆菌株: | DH5a |
培養條件: | 37度 |
■ 質粒屬性
質粒宿主: | 金黃色葡萄球菌 |
質粒用途: | 蛋白表達 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureu.pECTO a Gram negative/Gram positive shuttle vector derived from pMAD2 designed to integrate DNA sequences between SAOUHSC_00278 and SAOUHSC_00279 (NCTC8325 nomenclature HG003). It carries about 1kb of both sequences. A terminator introduced between the SAOUHSC_00278 and SAOUHSC_00279 sequences prevents polar effects associated with the expression of inserted genes.This a Gram negative/Gram positive shuttle vector with two specialized replicons and antibiotic markers. pBR322 replicon and ampicillin resistance for E. coli. pE194(ts) replicon and erythromycin resistance for S. aureus. In S. aureus, the plasmid propagates at T<30°C, it becomes a suicide vector at T>37°C.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK1904 pECTO金黃色葡萄球菌染色體基因異位互補質粒.txt
