■ 基本信息
別名: | Plasmid #79873;pAX01-dCas9 |
原核抗性: | Amp |
真核抗性: | Ery |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 枯草桿菌 |
■ 質粒屬性
質粒宿主: | 枯草桿菌 |
質粒用途: | 基因編輯 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
pJMP1質粒是一個枯草桿菌基因編輯質粒。Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA。dcas9 was PCR-amplified from pdCas9-bacteria (Addgene #44249) using primers containing BamHI-compatible BsaI sites, digested with BsaI, then ligated into plasmid pAX01 digested with BamHI to generate pJMP1。
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria.
Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA
Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. ?PubMed??Journal
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK2006 pJMP1枯草編輯質粒.txt
